Fig. 3.

hTAU^P301L overexpression increased AEA levels in the hippocampus. a Scheme of the ECS: main receptors, ligands, and enzymes involved in the endocannabinoid signalling pathway. b Analysis of AEA and 2-AG levels (pmol/g) measured by LC–MS on the ipsilateral and contralateral hippocampi from wild type mice injected with the AAV-hTAU^P301L, n = 8 samples ± SEM. c qRT-PCR determination of mRNA levels of Napepld, Dagla, and Dglb, involved in the synthesis of AEA and 2-AG, and d Faah and Mgll, involved in the degradation of AEA and 2-AG. Cox2 and Alox15, involved in other pathways. All measures were normalized by Tbp mRNA levels. n = 5 samples ± SEM. Asterisks denote significant differences *p < 0.05, according to Student's t-test