Fig. 6. K313 inhibits proliferation of leukemia cell lines via blocking PRMT1 activity. (A and B) K313 inhibited leukemic cell growth. (A) K313 was diluted serially and added to the MEG01, CMK, K562, and HEL cell cultures and cell viability was measured after 72 h treatment. The same amount of DMSO was added to the control. (B) The sensitivity curves were plotted to obtain IC₅₀ values. (C) Treatment of K313 decreased the arginine methylation level MEG01 cells. MEG01 cells were cultured with DMSO control or K313 for 24 h, and the cell extract samples were resolved by SDS-PAGE. PRMT1 activity was measured by the methylation level of substrate protein RBM15 by anti-methyl-RBM15 antibody and the total asymmetric arginine methylation level (α-Asym24 antibody). Equal loading was confirmed by Ponceau S staining.