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. Author manuscript; available in PMC: 2022 Apr 27.
Published in final edited form as: Anal Chem. 2021 Apr 12;93(16):6323–6328. doi: 10.1021/acs.analchem.1c00402

Figure 3.

Figure 3.

Changing the compensation voltage (CV) results in detection of a hidden population of proteins through improved signal-to-noise values. (A) Protein-level heatmap of CD3+ T cell proteins identified without FAIMS (8 technical replicates) or with FAIMS applying different CVs from −40 V to +30 V. CVs for injections analyzed with FAIMS are indicated at the bottom of the heatmap. (B) Mass spectrum of proteoform PFR295102 from UniProt accession P20292 (Arachidonate 5-lipoxygenase-activating protein) analyzed with FAIMS (top panel) and without FAIMS (lower panel) at same retention time. (C) Fragmentation map of PFR295102 showing 52% sequence coverage in the sample using FAIMS.