Fig. 4. Recent applications and advances in the phosphoramidite approach. A) Strategy of the 3-nitrophosphotyrosine based assay for phosphatase activity measurement.59 B) Fluorescence probe 1 for monitoring the phosphatase activity.15 C) Grb2-SH2 domain inhibitor 2.60 D) Structure of activators alternative to 1H-tetrazole. E) Synthesis of phosphocholinated tyrosine building block 3 for Fmoc SPPS.65.
