Fig. 4. IC₅₀ values of itaconate analogues against ICL1. Inhibition experiments contained 20 nM ICL1, 1 mM dl-isocitrate, 1 mM MgCl₂, 10 mM phenylhydrazine-HCl and varying concentration of itaconate analogues in 50 mM tris (pH 7.5). Errors represent standard deviations from four separate measurements.