Table 1.
Information from the Literature | Chemical | Sourceg | CASRN | Chemicalh Purity, % | Max Tested Conc., μM | Median % Inhibition at Max Conc. | IC20 (μM) | IC50 (μM) |
---|---|---|---|---|---|---|---|---|
Known or suspected inhibitors | 3-Nitro-L-tyrosine (MNT) a, b, c | AA | 621-44-3 | 99.2 | 200 | 100 | 0.01 | 0.04 |
3,5-L-Dibromotyrosine (DBT) a, d,e | TCI | 300-38-9 | 96.9 | 200 | 93 | 6.72 | 15.58 | |
3,5-L-Dinitrotyrosine (DNT) a, d | TCI | 502481-30-3 | 99.8 | 200 | 82 | 8.73 | 26.44 | |
Phloxine B f | SA | 18472-87-2 | NA | 200 | 88 | 17.27 | 28.70 | |
Triclosan f | SA | 3380-34-5 | 99.8 | 200 | 64 | 68.36 | 133.27 | |
Bromoxynil f | ToxCast | 1689-84-5 | 98.2 | 200 | −5 | --i | -- | |
Non-inhibitors | Bisphenol A e | SA | 80-05-7 | 99.9 | 200 | 4 | -- | -- |
Dibutyl phthalate e | SA | 84-74-2 | 99.6 | 200 | −6 | -- | -- | |
Genistein e | RPI | 446-72-0 | NA | 200 | −12 | -- | -- |
Chemicals used for development of the screening assay from stocks on hand at EPA/ORD/CCTE/GLTED, sources: AA = Alfa Aesar, TCI=TCI America, SA = Sigma-Aldrich, RPI=Research Products International, ToxCast = received on previous chemical source plate of ToxCast chemicals;
Purity of chemical as reported by the vendor. Not available (NA) for phloxine B or genistein.
--The chemical did not produce an inhibition curve, or it was not possible to calculate an IC20 or IC50 because the curve did not reach 20% or 50% inhibition.