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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Nat Immunol. 2020 Nov 9;22(1):67–73. doi: 10.1038/s41590-020-00828-7

Extended Data Fig. 5. Cytokine production by immune complex-stimulated monocytes.

Extended Data Fig. 5

Monocytes stimulated with highly afucosylated immune complexes made from both (a) patient IgG pools (IL8: p=0.0196 for 100μg/ml, p=0.0002 for 20μg/ml. IL10: p<0.0001 for 100μg/ml, p<0.0001 for 20μg/ml and p= 0.0019 for 4μg/ml. GM-CSF: p=0.0036 for 100μg/ml, p<0.0001 for 20μg/ml and p=0.003 for 4μg/ml) and (b) mAb 3022 (IL8: p=0.001 for 10μg/ml, p=0.0011 for 2μg/ml. IL10: p= 0.0045 for 10μg/ml, p=0.0112 for 2μg/ml and p= 0.0021 for 0.4μg/ml. GM-CSF: p=0.0007 for 10μg/ml, p<0.0001 for 2μg/ml and p=0.0036 for 0.4μg/ml) produced higher amounts of multiple proinflammatory cytokines as compared to immune complexes with low afucosylation. The assays were performed in duplicate with monocytes from three healthy donors and mean data and standard error of the mean (SEM) has been graphed. P-values between high and low afucosylated immune complexes at each antibody concentration were calculated by two-tailed paired t-tests. *P ≤ 0.05, **P ≤0.01, ***P ≤ 0.001, ****P ≤ 0.0001.