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Compound 3f (structure shown in the center) selectively binds NSD2 PWWP1. (A) Treatment with 3f increases the melting temperature of NSD2-PWWP1 in a DSLS assay. (B) 3f binds NSD2-PWWP1 in an SPR assay. (C) 3f only affects the melting temperature of NSD2-PWWP1 in a panel of 10 PWWP domains at 100 μM.
