Maufrais 2017.

Study characteristics
Methods	Randomised controlled trial, open‐label, cross‐over design
Participants	24 healthy young individuals (12 men, 12 women) of European descent with mean ± SD age of 23.3 ± 2.2 years and weight 62.9 ± 10.1 kg Exclusion criteria: Participants with a body mass index > 30 kg/m², competition athletes, and individuals with a daily exercise workload exceeding 60 minutes per day
Interventions	Participants ingested 1 of the following 4 drinks at a temperature of around 10°C (at a convenient pace over 5 minutes): 390 mL distilled water + 10 mL lemon juice 48 g sucrose + 10 mL lemon juice, diluted in distilled water up to a total volume of 400 mL Vodka (40% alcohol per volume, given at 1.28 mL/kg of body weight, providing 0.4 g alcohol/kg) + 10 mL lemon juice, diluted in distilled water up to 400 mL 48 g sucrose + 40% vodka (at 1.28 mL/kg) + 10 mL lemon juice, diluted in distilled water up to 400 mL
Outcomes	Heart rate Mean blood pressure Skin temperature Stroke volume Cardiac output Total peripheral resistance Measured before drinking and at 15, 30, 60, 90, and 120 minutes post drinking
Notes	None of the participants had any disease or were taking any medication affecting cardiovascular or autonomic regulation All participants were requested to avoid alcohol or caffeine for at least 24 hours before the test A light standardised breakfast provided by investigators, consisting of 1 mini‐pack of 33 cl of commercial light ice tea (33 kcal, 8 g carbohydrates/6.6 g sugar) and 2 cereal bars (total of 150 kcal, 39 g carbohydrates/12 g sugar), to avoid that consumption of alcohol in the same morning was done on an empty stomach Study authors chose type I error (α) of 0.05 and desired power (1‐β) of 0.80, suggesting that a total of 12 participants per gender would be required
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	Low risk	"random sequence generator (http://www.random.org/sequences/) where the session order was determined for 24 test subjects before the study started"
Allocation concealment (selection bias)	Unclear risk	"The test subjects were not allowed to know the order of their sessions in advance" Comment ‐ method of allocation concealment was not described
Blinding of participants and personnel (performance bias) All outcomes	High risk	Comment ‐ study was not blinded for participants and personnel
Blinding of outcome assessment (detection bias) All outcomes	High risk	Comment ‐ study was not blinded for outcome assessors
Incomplete outcome data (attrition bias) All outcomes	Unclear risk	Comment ‐ it is not explicitly mentioned whether all participants were included in the final analysis
Selective reporting (reporting bias) For systolic blood pressure (SBP)	High risk	Comment ‐ study authors did not report SBP
Selective reporting (reporting bias) For diastolic blood pressure (DBP)	High risk	Comment ‐ study authors did not report DBP
Selective reporting (reporting bias) For mean arterial blood pressure (MAP)	Low risk	Comment ‐ study authors reported MAP and SEM
Selective reporting (reporting bias) For heart rate (HR)	Low risk	Comment ‐ study authors reported HR and SEM
Other bias (conflict of interest, industry sponsorship)	Low risk	"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest" "This work was supported by the Swiss Foundation for Alcohol Research (project 276) and in part by the Swiss National Science Foundation"
Other bias (was the study registered in clinical trials.gov/ was the protocol available?)	High risk	Comment ‐ protocol was not registered and study identifier was not reported