Table 2.
Fluorophore uptake profile for three different membrane transporters of E. coli BW25113. Fluorescence (Signal Ratios) data are presented as the ratios of the fluorescence (median) from the three different E. coli knockout strains (∆yhjV, ∆yihN, and ∆tolC) over the fluorescence (median) from the reference strain ( E. coli BW25113). No difference between a KO strain and the reference would have had a ratio of 1. SD: standard deviation of the population, CI95: 95% confidence interval. The data for each knockout strain are sorted by incremental signal ratios
|
yhjV |
yihN |
tolC |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Fluor |
Median |
SD |
CI95 |
Fluor |
Median |
SD |
CI95 |
Fluor |
Median |
SD |
CI95 |
|
Thiazole orange |
0.29 |
0.06 |
[0.26–0.31] |
DiSC3(5) |
0.01 |
0 |
[0.01–0.01] |
Rhodamine 700 |
0.06 |
0.01 |
[0.05–0.06] |
|
ATTO Rho14 carboxy |
0.37 |
0.08 |
[0.34–0.41] |
Rhodamine 700 |
0.02 |
0 |
[0.02–0.02] |
DiSC3(5) |
0.07 |
0.01 |
[0.07–0.07] |
|
SYTO13 |
0.44 |
0.24 |
[0.32–0.55] |
2',7′-Dichlorofluorescein |
0.03 |
0.03 |
[0.01–0.04] |
Rhodamine 800 |
0.12 |
0.02 |
[0.11–0.13] |
|
SYBR Green I |
0.46 |
0.09 |
[0.43–0.49] |
CruzFluor 405 succinimidyl ester |
0.03 |
0.03 |
[0.01–0.05] |
Thiazole orange |
0.31 |
0.1 |
[0.26–0.36] |
|
Rhodamine 800 |
0.55 |
0.12 |
[0.49–0.61] |
Thiazole orange |
0.03 |
0.01 |
[0.02–0.03] |
DMAMP |
0.38 |
0.2 |
[0.31–0.45] |
|
Ethyl Red |
0.58 |
0.09 |
[0.53–0.62] |
NBDG |
0.04 |
0.04 |
[0.02–0.06] |
ATTO 490LS carboxy |
0.47 |
0.14 |
[0.4–0.54] |
|
Sennoside A |
0.6 |
0.07 |
[0.57–0.64] |
ATTO 490LS carboxy |
0.05 |
0.01 |
[0.04–0.05] |
ATTO Rho14 carboxy |
0.48 |
0.28 |
[0.35–0.62] |
|
Rhodamine B |
0.61 |
0.19 |
[0.54–0.68] |
SYBR Green I |
0.05 |
0.02 |
[0.04–0.06] |
SYBR Green I |
0.48 |
0.12 |
[0.42–0.54] |
|
Alizarin |
0.63 |
0.06 |
[0.61–0.65] |
ATTO Rho14 carboxy |
0.07 |
0.04 |
[0.05–0.09] |
Ethyl Red |
0.55 |
1.26 |
[-0.07–1.16] |
|
Amaranth |
0.68 |
0.13 |
[0.63–0.72] |
Ethyl Red |
0.08 |
0.02 |
[0.07–0.09] |
Trypan Blue |
0.68 |
0.22 |
[0.57–0.79] |
|
Rhodamine 700 |
0.69 |
0.17 |
[0.63–0.75] |
ATTO 488 carboxy |
0.09 |
0.02 |
[0.08–0.1] |
ATTO 633 carboxy |
0.69 |
0.07 |
[0.65–0.72] |
|
Cresol Red |
0.74 |
0.12 |
[0.7–0.78] |
ATTO 647 carboxy |
0.09 |
0.02 |
[0.08–0.11] |
2',7′-Dichlorofluorescein |
0.72 |
1.1 |
[0.18–1.26] |
|
DiSC3(5) |
0.75 |
0.2 |
[0.68–0.82] |
Rhodamine 800 |
0.1 |
0.02 |
[0.09–0.11] |
Oxazine 1 |
0.72 |
0.07 |
[0.69–0.75] |
|
Oxonol V |
0.75 |
0.15 |
[0.7–0.8] |
ATTO 430LS carboxy |
0.11 |
0.1 |
[0.05–0.16] |
NBDG |
0.75 |
1.08 |
[0.22–1.27] |
|
Phenol Red |
0.75 |
0.23 |
[0.67–0.83] |
7-Aminoactinomycin D |
0.12 |
0.12 |
[0.05–0.19] |
7-Aminoactinomycin D |
0.88 |
1.23 |
[0.28–1.49] |
|
Malachite Green |
0.76 |
0.17 |
[0.7–0.82] |
Ethidium bromide |
0.14 |
0.03 |
[0.13–0.16] |
BODIPY |
0.92 |
0.39 |
[0.73–1.11] |
|
Eosin Y |
0.77 |
0.05 |
[0.75–0.78] |
H2FDA |
0.16 |
0.05 |
[0.14–0.19] |
ATTO 430LS carboxy |
0.95 |
1.02 |
[0.45–1.45] |
|
Sulphorhodamine B |
0.77 |
0.22 |
[0.7–0.85] |
Doxorubicin |
0.17 |
0.02 |
[0.16–0.18] |
CruzFluor 405 succinimidyl ester |
1.07 |
1.12 |
[0.52–1.62] |
|
Brilliant Green |
0.79 |
0.12 |
[0.75–0.84] |
Trypan Blue |
0.17 |
0.04 |
[0.15–0.19] |
l-Kynurenine |
1.08 |
0.3 |
[0.94–1.22] |
|
Doxorubicin |
0.79 |
0.12 |
[0.75–0.84] |
ATTO 633 carboxy |
0.18 |
0.14 |
[0.1–0.26] |
Doxorubicin |
1.47 |
0.28 |
[1.33–1.61] |
|
Trypan Blue |
0.79 |
0.12 |
[0.74–0.83] |
DMAMP |
0.18 |
0.05 |
[0.16–0.21] |
Pyranine |
1.74 |
1.65 |
[0.94–2.55] |
|
l-Kynurenine |
0.8 |
0.12 |
[0.75–0.84] |
Celestine blue |
0.19 |
0.06 |
[0.16–0.23] |
Thionine |
1.89 |
0.74 |
[1.53–2.25] |
|
Pyronin Y |
0.8 |
0.17 |
[0.74–0.85] |
Oxazine 1 |
0.19 |
0.06 |
[0.16–0.23] |
Alizarin |
1.92 |
0.1 |
[1.87–1.96] |
|
Celestine blue |
0.82 |
0.05 |
[0.8–0.85] |
Calcein |
0.2 |
0.03 |
[0.18–0.22] |
Methylene blue |
1.97 |
0.09 |
[1.93–2.01] |
|
ATTO 488 carboxy |
0.83 |
0.18 |
[0.77–0.9] |
Cresol Red |
0.26 |
0.07 |
[0.23–0.3] |
Celestine blue |
13.82 |
6.56 |
[10.61–17.03] |
|
Thionine |
0.84 |
0.1 |
[0.81–0.88] |
Eosin Y |
0.3 |
0.06 |
[0.27–0.34] |
Azure C |
2.2 |
0.61 |
[1.9–2.5] |
|
Hoechst 33 342 |
0.85 |
0.05 |
[0.83–0.87] |
Mithramycin A |
0.3 |
0.02 |
[0.28–0.31] |
Phenol Red |
2.21 |
0.12 |
[2.15–2.26] |
|
Neutral Red |
0.85 |
0.09 |
[0.81–0.88] |
Riboflavin |
0.3 |
0.04 |
[0.28–0.32] |
Safranine O |
2.27 |
0.08 |
[2.23–2.31] |
|
Safranine O |
0.85 |
0.14 |
[0.79–0.9] |
Acridine orange |
0.31 |
0.05 |
[0.28–0.34] |
Sulphorhodamine B |
2.29 |
0.26 |
[2.17–2.42] |
|
Mithramycin A |
0.86 |
0.18 |
[0.8–0.92] |
5-Carboxyfluorescein |
0.32 |
0.04 |
[0.29–0.34] |
Oxazine 170 |
2.4 |
0.59 |
[2.11–2.69] |
|
9-aminoacridine |
0.88 |
0.06 |
[0.86–0.9] |
Amaranth |
0.32 |
0.08 |
[0.27–0.36] |
5-Carboxyfluorescein |
2.44 |
4.99 |
[-0.01–4.89] |
|
Azure B |
0.88 |
0.11 |
[0.84–0.92] |
Pyronin Y |
0.33 |
0.05 |
[0.3–0.36] |
Azure B |
2.53 |
0.17 |
[2.45–2.62] |
|
DMAMP |
0.88 |
0.14 |
[0.83–0.93] |
Azure A |
0.34 |
0.02 |
[0.33–0.35] |
Azure A |
2.61 |
0.18 |
[2.52–2.7] |
|
ASA |
0.9 |
0.02 |
[0.89–0.91] |
Sennoside A |
0.37 |
0.04 |
[0.35–0.39] |
Oxonol V |
2.63 |
0.35 |
[2.45–2.8] |
|
ATTO 647 carboxy |
0.9 |
0.17 |
[0.84–0.95] |
Phenol Red |
0.41 |
0.03 |
[0.4–0.43] |
Cresol Red |
2.67 |
0.27 |
[2.54–2.81] |
|
Berberine |
0.9 |
0.08 |
[0.87–0.93] |
Thionine |
0.42 |
0.06 |
[0.39–0.46] |
Sennoside A |
2.93 |
0.64 |
[2.62–3.25] |
|
5-Carboxyfluorescein |
0.91 |
0.09 |
[0.88–0.94] |
l-Kynurenine |
0.43 |
0.12 |
[0.37–0.5] |
Riboflavin |
3.21 |
0.12 |
[3.15–3.27] |
|
ATTO 490LS carboxy |
0.91 |
0.13 |
[0.85–0.97] |
Malachite Green |
0.43 |
0.05 |
[0.4–0.46] |
Ethidium bromide |
3.33 |
0.88 |
[2.9–3.76] |
|
H2FDA |
0.91 |
0.12 |
[0.87–0.95] |
Azure B |
0.44 |
0.16 |
[0.35–0.53] |
ATTO 647 carboxy |
3.44 |
0.92 |
[2.99–3.89] |
|
Acridine orange |
0.92 |
0.17 |
[0.86–0.98] |
Oxazine 170 |
0.44 |
0.04 |
[0.42–0.47] |
ATTO 488 carboxy |
3.56 |
0.34 |
[3.39–3.72] |
|
Methylene blue |
0.92 |
0.17 |
[0.86–0.98] |
Safranine O |
0.45 |
0.03 |
[0.43–0.46] |
Acridine orange |
3.8 |
1 |
[3.31–4.29] |
|
Riboflavin |
0.92 |
0.12 |
[0.88–0.96] |
Brilliant Green |
0.46 |
0.07 |
[0.42–0.5] |
Mithramycin A |
4.05 |
0.75 |
[3.68–4.42] |
|
Azure A |
0.93 |
0.15 |
[0.88–0.98] |
Methylene blue |
0.47 |
0.1 |
[0.42–0.53] |
Eosin Y |
4.5 |
0.87 |
[4.07–4.93] |
|
Calcein |
0.95 |
0.13 |
[0.9–0.99] |
Azure C |
0.48 |
0.15 |
[0.4–0.57] |
Brilliant Green |
4.78 |
0.26 |
[4.65–4.91] |
|
Ethidium bromide |
0.95 |
0.05 |
[0.93–0.96] |
Pyranine |
0.48 |
0.02 |
[0.47–0.49] |
Malachite Green |
4.97 |
0.45 |
[4.75–5.19] |
|
Fluorescein |
0.95 |
0.1 |
[0.91–0.98] |
Oxonol V |
0.51 |
0.09 |
[0.47–0.56] |
Amaranth |
5.02 |
0.74 |
[4.66–5.39] |
|
Azure C |
0.97 |
0.04 |
[0.95–0.98] |
Neutral Red |
0.52 |
0.1 |
[0.47–0.58] |
Neutral Red |
5.03 |
0.2 |
[4.93–5.13] |
|
Oxazine 1 |
0.98 |
0.14 |
[0.93–1.03] |
9-aminoacridine |
0.57 |
0.03 |
[0.56–0.59] |
9-aminoacridine |
5.18 |
0.43 |
[4.97–5.4] |
|
Oxazine 170 |
0.99 |
0.15 |
[0.94–1.04] |
BODIPY |
0.57 |
0.08 |
[0.53–0.62] |
Calcein |
5.26 |
0.2 |
[5.16–5.36] |
|
ATTO 633 carboxy |
1 |
0.17 |
[0.92–1.09] |
Sulphorhodamine B |
0.6 |
0.12 |
[0.53–0.67] |
Pyronin Y |
5.27 |
1.27 |
[4.65–5.9] |
|
CPDPP |
1.01 |
0.04 |
[0.99–1.03] |
ASA |
0.66 |
0.03 |
[0.64–0.67] |
Fluorescein |
5.62 |
0.2 |
[5.52–5.72] |
|
Pyranine |
1.01 |
0.15 |
[0.96–1.07] |
Fluorescein |
0.66 |
0.06 |
[0.62–0.69] |
H2FDA |
5.86 |
0.97 |
[5.39–6.34] |
|
DPDPP |
1.04 |
0.2 |
[0.94–1.14] |
Alizarin |
0.73 |
0.14 |
[0.65–0.81] |
Hoechst 33 342 |
6.1 |
0.51 |
[5.85–6.35] |
|
BODIPY |
1.19 |
0.05 |
[1.17–1.21] |
Rhodamine B |
0.79 |
0.18 |
[0.69–0.9] |
Berberine |
6.36 |
0.61 |
[6.06–6.66] |
|
Berberine |
0.96 |
0.12 |
[0.89–1.03] |
ASA |
6.51 |
0.42 |
[6.3–6.71] |
||||
|
Hoechst 33 342 |
1.01 |
0.12 |
[0.94–1.08] |
Rhodamine B |
6.8 |
0.49 |
[6.56–7.04] |
||||