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. 2021 May 18;8:64–71. doi: 10.18632/oncoscience.535

Figure 3. Constitutive activation of MEK5 downregulates CDH1 expression and enhances TNBC cell migration.

Figure 3

(A) GSEA analysis of RNA sequencing of MDA-MB-231-MEK5-ca cells compared to parental controls demonstrating upregulation of EMT genes in MEK5-ca cells. qPCR for EMT markers in (B) MDA-MB-231 or (C) Hs-578T-vector and -MEK5-ca cells (C) Western blot of the epithelial marker CDH1 in MEK5-ca cells. (D) Transwell migration assay for MDA-MB-231- or Hs-578T-parental and -ERK5-ko cells. After 24 hours, migrated cells were fixed, stained with crystal violet, and quantified. Bars represent average number of migrated cells normalized to parental cells (set to 100%) ± SEM of triplicate experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.