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. 2021 May 18;8:64–71. doi: 10.18632/oncoscience.535

Figure 5. MEK5-ca upregulates cell cycle pathways but does not affect cell proliferation.

Figure 5

(A) GSEA analysis of RNA sequencing of MDA-MB-231-MEK5-ca cells compared to parental controls demonstrating upregulation of (B) cell cycle related genes in MEK5-ca cells. (C) IF staining of Ki-67 in MDA-MB-231-vector and -MEK5-ca cells, viewed at 200x. (D) Representative images were taken per well and percentage of Ki-67-positive cells relative to total (DAPI-positive) cells was calculated. Bars represent mean % of Ki-67 positive cells ± SEM of triplicate experiments.