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. 2021 May 13;59(1):41. doi: 10.3892/ijo.2021.5221

Figure 4.

Figure 4

PCNA-I1S (P-I1S) and T2AA inhibit AR transcriptional activity. (A) LNCaP or (B, C and E) 22Rv1 cells (105 cells/well) in 12-well plates in stripped medium containing 10% of charcoal-dextran treated FBS (sFBS) were transfected with PSA-luc or p21(-215)-luc (0.2 µg/well), and treated with PCNA-I1S at (A) 0.5 µM, (B) various concentrations, (C) 1 µM, or (E) T2AA for 24 h in the absence or presence of 10 nM DHT. (D) PC-3 cells transfected with PSA-luc (0.2 µg/well) without or with AR expression vector CMV-AR (0.2 µg/well) were treated with 1 µM PCNA-I1S in the absence or presence of 10 nM DHT for 24 h. Subsequently, luciferase activity was assessed. **P<0.01, PCNA-I1S plus DHT vs. DHT alone (A-D) and T2AA plus DHT vs. DHT alone. PCNA, proliferating cell nuclear antigen; DHT, dihydrotestosterone.