Figure 7.

R9-AR-PIP inhibits the expression of AR target genes and cell growth. (A) Determination of the affinity of R9-AR-PIP to GFP-PCNA and GFP by thermophoresis with temperature jump. (B) Cells were treated without or with 20 µM R9-AR-PIP in the absence or presence of 10 nM DHT for 48 h, followed by western blot analysis. (C) PC-3 cells transfected with PSA-luc without or with CMV-AR were treated with R9-AR-PIP in the presence of 10 nM DHT for 24 h, followed by luciferase assay. (D) Cells were treated for five days with R9-AR-PIP in the absence or presence of 10 nM DHT, followed by MTT assay. ^*P<0.05 and ^**P<0.01, R9-AR-PIP vs. control without the small peptide treatment.