Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | SLC6A3 | Uniprot | Uniprot Q01959 | Encodes hDAT protein |
| Gene (Aquifex aeolicus) | LeuT | Uniprot | Uniprot O67854 | Encodes LeuT protein |
| Strain, strain background (Escherichia coli) | C43 DE3 F– ompT gal dcm hsdSB(rB- mB-)(DE3) |
Lucigen | ||
| Genetic reagent (D. melanogaster) | W1118 | Bloomington Stock Center | BI 6326 | |
| Genetic reagent (D. melanogaster) | TH-GAL4 | Bloomington Stock Center | BI 8848 | |
| Genetic reagent (D. melanogaster) | DATMB07315 | Bloomington Stock Center | BI 25547 | |
| Genetic reagent (D. melanogaster) | UAS-mCherry | Kyoto Stock Center | 109594 | |
| Genetic reagent (D. melanogaster) | M[vas-int.Dm]ZH-2A; M[3xP3-RFP.attP']ZH-22A | Rainbow Transgenic Flies | 24481 | |
| Genetic reagent (D. melanogaster) | DATfmn | Gift from Dr. K. Kume | ||
| Cell line (Homo sapiens) | HEK | |||
| Antibody | Anti-DAT(Rat monoclonal) | Millipore | MAB369 | WB (1:1000) |
| Antibody | Anti- β-actin (Mouse monoclonal) | Sigma-Aldrich | A5441 | WB (1:5000) |
| Antibody | Anti- Na-K ATPase | Developmental Studies Hybridoma Bank | a5 | WB (1:100) |
| Antibody | Anti- TH | Millipore | AB152 | IHC (1:200) |
| Antibody | Anti- Bruchpilot | Developmental Studies Hybridoma Bank | nc82 | IHC (1:50) |
| Recombinant DNA reagent | pEGFP (plasmid) | Clonetech | ||
| Recombinant DNA reagent | pET16b (plasmid) | Novagen | ||
| Peptide, recombinant protein | LeuT | This paper | Purified from C43 (DE3) E. coli | |
| Peptide, recombinant protein | LeuT R375A | This paper | Purified from C43 (DE3) E. coli | |
| Peptide, recombinant protein | LeuT R375C | This paper | Purified from C43 (DE3) E. coli | |
| Peptide, recombinant protein | LeuT R375D | This paper | Purified from C43 (DE3) E. coli | |
| Chemical compound, drug | [3H]dopamine | PerkinElmer Life Sciences | NET673250UC | |
| Chemical compound, drug | Sulfo-NHS-SS-biotin | Fisher | PG82078 | |
| Other | Fugene-6 | Roche Molecular Biochemicals |