Figure 3. Automated detection of mutant SOD1 aggregates.
A. Compound microscope fluorescence micrographs of the same G85R SOD1 cells using GFP filter set to image the SOD1-YFP aggregates and the TRITC filter set to image the Image-iT WGA plasma membrane stain. B. Aggregate detection with the Cellomics Arrayscan 3.5 using spot detector software to image cells with Image-iT in channel 1 and SOD1-YFP aggregates in channel 2. Data are expressed as spot count (aggregates) per object (cell).