Extended Data Fig. 1. Timeline of behavioural training, presented stimuli and learning performance of individual mice for both categorization rules.
a, Timeline showing behavioural training stages, the number of training sessions that mice spent in each stage (min–max) and the imaging sessions (T1–T8). b, Stimuli used for category training, aligned to the stages shown in a. The scheme shows stimuli for mice that were trained on the spatial frequency rule first, and the orientation rule second. c, As in b, but for mice trained on the orientation rule first. d, Per mouse, the learning curve for training on rule 1. Blue curve denotes single session d′. Orange curve denotes sigmoid fit of d′. Arrows indicate imaging time points T2, T3 and T4. e, As in d, but for rule 2. Arrows indicate imaging time points T6 and T7. f, Parameters describing the fitted sigmoidal curves, comparing rules 1 and 2. Left, maximum. P = 0.71, two-tailed paired-samples t-test (n = 11). Middle, slope. P = 1.9 × 10−5, two-tailed paired-samples t-test (n = 11). Right, inflection point. P = 9.3 × 10−6, two-tailed paired-samples t-test (n = 11). g, Root-mean-square error (RMSE) of sigmoid fit. P = 0.013, two-tailed paired-samples t-test (n = 11). h, d′ of all mice comparing naive and learned discrimination of the initial two stimuli for the first rule (left) and the second rule (right). Black line indicates the mean across all mice, grey lines represent data of individual mice.