Figure 2:

Modified simplex optimization. The optimization of the dynamic exclusion window and number of MS² scan events is represented graphically with an overlaid heatmap to depict the relative number of identified neuropeptides under each condition. The initial vertices are denoted by V₁, V₂, and V₃; the subsequent simplexes contain points reflected away from the point of worst performance (R), an elongated reflection (E), a contracted reflection (CR), and the contracted reflection closer to the direction of the worst point (CW).