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. 2021 Apr 20;296:100681. doi: 10.1016/j.jbc.2021.100681

Figure 1.

Figure 1

Class-I release factor-mediated steps of translation termination, which govern the speed and accuracy of the process.A and B, represent compact and open conformations of RFs, respectively, with the domains I, II, III, and IV colored in yellow, green, purple, and pink. In the closed state, the distance between the GGQ and the PXT/SPF motifs is 25 Å, which increases to 75 Å in the open state on the ribosome. C, the scheme describes the main events of translation termination where the RFs bind to the ribosome with an empty A-site harboring a stop codon in the compact state. The RFs undergo “compact to open” transition upon recognition of the stop codon and accommodation in the A-site. Lastly, the peptide is released from the peptidyl tRNA by participation of the GGQ motif of the RFs in the open state. The first two steps contribute to accuracy of the process, whereas the catalytic peptide release state governs speed of translation termination.