Table 2.
Accuracy of stop codon recognition by methylated and unmethylated release factors
| Release factor 2 | Cognate versus Cognate |
←Cognate versus near-cognate→ |
|||
|---|---|---|---|---|---|
| A(UAA/UGA) | A(UAA/UAG) | A(UAA/UGG) | Release factor 1 | A(UAA/UGA) | |
| mRF2 | 0.74 ± 0.017 | 2625 ± 203.13 | 4200 ± 116.00 | mRF1 | 2300 ± 153.33 |
| RF2 | 0.83 ± 0.0004 | 1000 ± 20.00 | 1000 ± 30.00 | RF1 | 1340 ± 107.20 |
| mRF2/RF2 | 0.9 | 2.6 | 4.2 | mRF1/RF1 | 1.72 |
The data are presented as “A values.” A = (kcat/KM) cognate/(kcat/KM) near-cognate with SEM estimated from at least three independent sets of experiments.