Table 3.

Kinetic values of FABP3 binding to αSyn proteins, αSyn-derived peptides, and arachidonic acid, derived from the experiments shown in Figures 3 and 4

Sample	Kd (nM)	kon (M−1・s−1)	koff (s−1)
FABP3 versus αSyn	7.2	6.4 × 105	4.6 × 10−3
FABP3 versus arachidonic acid	6.8	0.8 × 105	0.6 × 10−3
FABP3 versus αSynΔ130-140	N.D.	N.D.	N.D.
FABP3 versus αSyn130-140CF	8.1	3.0 × 105	2.4 × 10−3
FABP3 versus αSynY133A/Y136A	61.7	0.9 × 105	5.5 × 10−3
FABP3 versus αSynP2-10	N.D.	N.D.	N.D.
FABP3 versus αSynP73-96	N.D.	N.D.	N.D.
FABP3 versus αSynP130-140	6.1	2.0 × 105	1.2 × 10−3
FABP3 F16S versus αSyn	N.D.	N.D.	N.D.
FABP3 F16S versus αSynP130-140	N.D.	N.D.	N.D.
FABP3 F16S versus arachidonic acid	2090	0.02 × 105	4.7 × 10−3
FABP3 versus αSynP130-140Y133F	2.0	7.5 × 105	1.5 × 10−3
FABP3 versus αSynP130-140Y136F	7.7	4.8 × 105	3.7 × 10−3
FABP3 versus αSynP130-140Y136W	6.3	3.8 × 105	2.4 × 10−3
FABP3 versus αSynP130-140 Y133F/Y136F	2.1	5.4 × 105	1.1 × 10−3
FABP3 versus αSynP130-140 Y133F/Y136W	6.1	3.2 × 105	1.9 × 10−3
FABP3 versus αSynP130-140Y133W/Y136W	6.2	2.4 × 105	1.5 × 10−3

αSyn, α-synuclein; FABP3, fatty acid binding protein 3; N.D., not determined.