. 2021 May 18;11(6):274. doi: 10.1007/s13205-021-02810-y

Table 4.

Molecular markers of R genes/QTLs for MAS against oomycetes pathogens

Pathogen	Gene/QTL	Marker/primer	Marker type	Primer sequence (5′ → 3′)	References
Phytophthora infestans	R1	R1-1205	SCAR	CACTCGTGACATATCCTCACTA	Sokolova et al. (2011)
		R1-1205	SCAR	GTAGTACCTATCTTATTTCTGCAAGAAT	Sokolova et al. (2011)
		BA47f2	SCAR	TAACCAACATTATCTTCTTTGCC	Gebhardt et al. (2004)
		BA47f2	SCAR	GAATTTGGAGAGGGGTTTGCTG	Gebhardt et al. (2004)
		CosA	SCAR	CTCATTCAAAATCAGTTTTGATC	Gebhardt et al. (2004)
		CosA	SCAR	GAATGTTGAATCTTTTTGTGAAGG	Gebhardt et al. (2004)
		R1F/R (76-2sf2/76-2SR)	AS	CACTCGTGACATATCCTCACTA	Ballvora et al. (2002)
		R1F/R (76-2sf2/76-2SR)	AS	CAACCCTGGCATGCCACG	Ballvora et al. (2002)
		GP76	SCAR	ATGAAGCAACACTGATGCAA	Oberhagemann et al. (1999)
		GP76	SCAR	TTCTCCAATGAACGCAAACT	Oberhagemann et al. (1999)
		SPUD237 (AluI)	CAPS	TTCCTGCTGATACTGACT	De Jong et al. (1997)
		SPUD237 (AluI)	CAPS	AGAAAACC AGCCAAGGAAAAGCTAGCATCCAAG	De Jong et al. (1997)
		GP21 (AluI)	CAPS	AGTGAGCCAGCATAGCATTACTTG	De Jong et al. (1997)
		GP21 (AluI)	CAPS	GGTTGGTGGCCTATTAGCCATGC	De Jong et al. (1997)
		GP179	SCAR	GGTTTTAGTGATTGTGCTGC	Meksem et al. (1995)
		GP179	SCAR	AATTTCAGACGAGTAGGCACT	Meksem et al. (1995)
	R3 (R3a & R3b)	R3-1380	SCAR	TCCGACATGTATTGATCTCCCTG	Sokolova et al. (2011)
		R3-1380	SCAR	AGCCACTTCAGCTTCTTACAGTAGG	Sokolova et al. (2011)
		SHa-F/ SHa-R	AS	ATCGTTGTCATGCTATGAGATTGTT	Huang et al. (2005)
		SHa-F/ SHa-R	AS	CTTCAAGGTAGTGGGCAGTATGCTT	Huang et al. (2005)
		R3bF4/R3bR5	AS	GTCGATGAATGCTATGTTTCTCGAGA	Rietman (2011)
		R3bF4/R3bR5	AS	ACCAGTTTCTTGCAATTCCAGATTG	Rietman (2011)
	RB/Rpi-blb1	RB-629/638	SCAR	AATCAAATTATCCACCCCAA	Sokolova et al. (2011)
		RB-629/638	SCAR	CTTTTAAATCAAGTATTGGGAGGACTGAAAGGT	Sokolova et al. (2011)
		RB-1223	SCAR	ATGGCTGAAGCTTTCATTCAAGTTCTG	Pankin et al. (2011)
		RB-1223	SCAR	CAAGTATTGGGAGGACTGAAAGGT	Pankin et al. (2011)
		CT88 (Primer1/primer 1′)	SCAR	CACGAGTGCCCTTTTCTGAC	Colton et al. (2006)
		CT88 (Primer1/primer 1′)	SCAR	ACAATTGAATTTTTAGACTT	Colton et al. (2006)
	Rpi-abpt	R2-F1/R2-R3	AS	GCTCCTGATACGATCCATG	Kim et al. (2012)
		R2-F1/R2-R3	AS	ACGGCTTCTTGAATGAA	Kim et al. (2012)
		Th2	CAPS	AGGATTTCAGTATGTCTCG	Park et al. (2005)
		Th2	CAPS	TCCATTGTTGATTGCCCCT	Park et al. (2005)
	Rpi-ber1	CT214 (DdeI)	CAPS	GAACGCGAAAGAGTGCTGATAG	Tan et al. (2010)
		CT214 (DdeI)	CAPS	CCCGCTGCCTATGGAGAGT	Tan et al. (2010)
		TG63(Bme1390I)	CAPS	TCCAATTGCCAGACGAA	Tan et al. (2010)
		TG63(Bme1390I)	CAPS	TAGAGAAGGCCCTTGTAAGTTT	Tan et al. (2010)
		Q133	SCAR	ATCATCTCCTCAAAGAATCAAG	Tan et al. (2010)
		Q133	SCAR	ATCTCCCCATTGACAACCAA	Tan et al. (2010)
	Rpi-mcd1	TG339 (MnlI)	CAPS	GCTGAACGCTATGAGGAGATG	Tan et al. (2010)
	Rpi-mcd1	TG339 (MnlI)	CAPS	TGAGGTTATCACGCAGAAGTTG	Tan et al. (2010)
	Rpi-phu1	GP94 (OPB07 + TG/GT)	RAPD	GAAACGGGTG + TG/GT	Sliwka et al. (2006)
	QTL_phu-stn	OPA17	RAPD	GACCGCTTGT	Wickramasinghe et al. (2009)
		OPA03	RAPD	AGTCAGCCAC	Wickramasinghe et al. (2009)
		GP198F/R	SCAR	GTAATTTGCGAGGAAGGAGAAG	Wickramasinghe et al. (2009)
		GP198F/R	SCAR	TCACTTTGGTGCTTCTGTCG	Wickramasinghe et al. (2009)
		GP198F-1/R	AS	TTTGCTTACTCTTGTTGTATG	Wickramasinghe et al. (2009)
		GP198F-1/R	AS	TCACTTTGGTGCTTCTGTCG	Wickramasinghe et al. (2009)
	Rpi-sto1	Ssto-448	SCAR	GTGGAACGCCGTCCATCCTTAG	Sokolova et al. (2011)
	Rpi-sto1	Ssto-448	SCAR	TGCATAGGTGGTTAGATGTATGTTTGATTA	Sokolova et al. (2011)
	Rpi-avl1	N2527	AS	GAAACACAGGGGAATATTCACC	Verzaux (2010)
	Rpi-avl1	N2527	AS	CCATRTCTTGWATTAAGTCATGC	Verzaux (2010)
	Rpi-cap1	CP58 (MspI)	CAPS	ATGTATGGTTCGGGATCTGG	Jacobs et al. (2010)
	Rpi-cap1	CP58 (MspI)	CAPS	TTAGCACCAACAGCTCCTCT	Jacobs et al. (2010)
	Rpi-dlc1	GP101 (AluI)	CAPS	GGCATTTCTATGGTATCAGAG	Golas et al. (2010)
		GP101 (AluI)	CAPS	GCTTAACATGCAAAGGTTAAA	Golas et al. (2010)
		S1d5-a	AS	CGCCTCTTTCTCTGAATTTC	Golas et al. (2010)
		S1d5-a	AS	GATCTGGGATGGTCCATTC	Golas et al. (2010)
	Rpi-mcq1	TG328 (AluI)	CAPS	AATTAAATGGAGGGGGTATC	Smilde et al. (2005)
	Rpi-mcq1	TG328 (AluI)	CAPS	GTAGTATTCTAGTTAAACTACC	Smilde et al. (2005)
	Rpi-snk1.1 and Rpi-snk1.2	Th21 (MboI)	CAPS	ATTCAAAATTCTAGTTCCGCC	Jacobs et al. (2010)
	Rpi-snk1.1 and Rpi-snk1.2	Th21 (MboI)	CAPS	AACGGCAAAAAAGCACCAC	Jacobs et al. (2010)
	Rpi-ver1	CD67 (HpyCH4I, SsiI)	CAPS	CCCCTGCAAATCCGTACATA	Jacobs et al. (2010)
	Rpi-ver1	CD67 (HpyCH4I, SsiI)	CAPS	CCATACGAGTTGAGGGATCG	Jacobs et al. (2010)
	Rpi-vnt1.1	TG35 (HhaI/XapI)	CAPS	CACGGAGACTAAGATTCAGG	Pel et al. (2009)
TAAAGGTGATGCTGATGGGG	Rpi-vnt1.1	TG35 (HhaI/XapI)	CAPS		Pel et al. (2009)
Rpi-vnt1.3	NBS3B	AS	CCTTCCTCATCCTCACATTTAG	Pel et al. (2009)
Rpi-vnt1.3	NBS3B	AS	GCATGCCAACTATTGAAACAAC	Pel et al. (2009)
R2	R2-800	PCR	TACTAACCTTTTCCTAGATG	Mori et al. (2011)
R2	R2-800	PCR	AAAACTTTCACGCACCCATAGGA	Mori et al. (2011)
R8	R8	PCR	AACAAGAGATGAATTAAGTCGGTAGC	Vossen et al. (2016)
R8	R8	PCR	AGAACTTTCTCACAGCTTTT	Vossen et al. (2016)
Rpi2	SpCP56	SCAR	GGCATATTACTTGAATCCAATA	Yang et al. (2017)
	SpCP56	SCAR	AGCAGAGGAGCACCATACT	Yang et al. (2017)
	SpGot2-1	CAPS Msp1	AAGCGCTTTTGTCCTGATTC	Yang et al.(2017)
	SpGot2-1	CAPS Msp1	TTCCCCGAGCCATAGAGTT	Yang et al.(2017)
	SpGro1	CAPS Taq1	GAGTTGAGGTGGCTTGATTGG	Yang et al. (2017)
	SpGro1	CAPS Taq1	CAACATTGGATATTTGGTTAGTTGA	Yang et al. (2017)
	SpCT226	CAPS Tru1	GGCATATTACTTGAATCCAATA	Yang et al. (2017)
	SpCT226	CAPS Tru1	AGCAGAGGAGCACCATACT	Yang et al. (2017)
	SpT1756	CAPS Msp1	GTTGATGCCTGTGTCGTCGTT	Yang et al. (2017)
	SpT1756	CAPS Msp1	TAATGCGTCCTTCTTCGTCAATCT	Yang et al. (2017)
	SpAFLP2	CAPS Tail1	CAACAAATAGGCATGTGAAGAAC	Yang et al. (2017)
	SpAFLP2	CAPS Tail1	GACCCCACCAACCCAAAAAGAC	Yang et al. (2017)
	SpTG572	SCAR	TCCTTGATCCCCTCCCTTTGGTGTGA	Yang et al. (2017)
	SpTG572	SCAR	TCATTTCCAGCCAAGCTATTTAC	Yang et al. (2017)
	SpAFLP1	CAPS Taq1	TCAGAGGACACGAGATTACG	Yang et al.(2017)
	SpAFLP1	CAPS Taq1	ATACCATCCTCATTTTCT TTGTTA	Yang et al.(2017)
	SpAL21	CAPS Taq1	TTTTTGGGCTTGTCCTCTGC	Yang et al. (2017)
	SpAL21		GATCCCCTTCTGCGTGGT	Yang et al. (2017)
	SpGP127	CAPS Trul1	GGTGATCGACTGGAACGTCT	Yang et al. (2017)
	SpGP127	CAPS Trul1	CTGCAGATTCCTCGCATACT	Yang et al. (2017)
	SpTG20	CAPS Rsa1	GAGTGGCATGGTGTGGCAGTTCT	Yang et al. (2017)
	SpTG20	CAPS Rsa1	TCTTTGTGGGGTTTTGGAGTTTTT	Yang et al. (2017)
R9a	CDP^Hero33	CDP/HaeIII	RRAGATTCAGCCATKGARATTAAGAAA	Jo et al. (2011)
	CDP^Tm22	CDP/MseI	GCCAAATAGTATTGTCAAGCTC	Jo et al. (2011)
	CDP^Tm26	CDP/MseI	CATTTCTCTCTGGAGCCAATC	Verzaux (2010)
	CDP^Tm27	CDP/MseI	CAAGTTTGTCGCAGAGATTGA	Verzaux (2010)
	CDP^Sw58	CDP/MseI	AAGGATGCGACCGTATTGACCTCAT	Jo et al. (2015)
	CDP^Sw59	CDP/MseI	AAGGATGCGACCGTATTGACCTCAT	Jo et al.(2015)
	CDP^Sw510	CDP/MseI	AAGGATGCGACCGTATTGACCTCAT	Jo et al. (2015)
	184–81	CAPS/RsaI	CCACCGTATGCTCCGCCGTC	Jo et al. (2011)
	184–81	CAPS/RsaI	GTTCCACTTAGCCTTGTCTTGCTCA	Jo et al. (2011)
	Stm1021	SSR	GGAGTCAAAGTTTGCTCACATC	Collins et al. (1999)
	Stm1021	SSR	CACCCTCAACCCCCATATC	Collins et al. (1999)
Phytophthora sojae	Rps		SSR	TTGTGATCGTTTGGGATGAG	Li et al. (2017)
				GGAAAAGGATGCATAGCAAAA
				GGTAGATCCAGGAGCTTGAGTCAG
				GCGCATCTCACTGCACTTGATTTT
				GCGACGCGCTAGTCTTATTT
				GCGGATGGCTTTTACTTT
				AAAACCTCGTTCCCACTGTT
				TCTTCCTTGGACTCCTCGAA
	Rps 9		SSR	GGTAGATCCAGGAGCTTGAGTCAG	Wu et al. (2011)
	Rps 9		SSR	GCGCATCTCACTGCACTTGATTTT	Wu et al. (2011)
	Rps1		SSR	GAAATGCCCAGAAAAACCTAATAAC	Demirbas et al. (2001)
				TGAAGCAACAAAATAGAGGAATAGAG
				GCGCTATTCCTATCACAACACA
				TAGGGTTGTCACTGTTTTGTTCTTA
				CATGCATATTGACTTCATTATT
				CCAAGCGGGTGAAGAGGTTTTT
				GCGCCCAAACCTATTAAGGTATGAACA
				GCGGGTCAGAAGATGCTACCAAACTCT
	Rps2		SSR	GCGCTATCCGATCCATATGTG
	Rps2			TGATTTCGCTAGGTAAAATCA
	Rps3			GGGTTATCCTCCCCAATA
	Rps3			ATATGGGATGATAAGGTGAAA
	Rps4			GCGAATACATAAAACTCAAATTCAAATCATA
	Rps4			GCGTTCTATAAATTTCATTCATAGTTTCAAT
	Rps6			CGCGATCATGTCTCTG
	Rps6			GGGAGTTGGTGTTTTCTTGTG
Phytophthora capsici	Phyto 5.2		SCAR	CCA TAA GGG TTGGTA AAT TTA CAA AG	Quirin et al. (2005)
	Phyto 5.2		SCAR	TCG AGAGATAAT TCA GAT AGTATA ATC	Quirin et al. (2005)
	Phyto 5.2		SSR	CAGCGTTCTATCGTCTCAAATG	Minamiyama et al. (2007)
	Phyto 5.2		SSR	TTGACAAACCAGAAATTGATCG	Minamiyama et al. (2007)
	CaPhyto		SSR	TCCAGCCATCCATTATTTCAT	Wang et al. (2016)
	CaPhyto		SSR	ATCCCGAACTGCCAATAATTA	Wang et al. (2016)
	Phyto5SAR		SNP	TTGATAGCCCCTGGTAAAGA	Liu et al. (2014)
	Phyto5SAR		SNP	GTGGTGATATTCAAACGGG	Liu et al. (2014)
	PhR 10		SSR	CAATCCAAACAAGTCCTAAG	Xu et al. (2016)
	PhR 10		SSR	GGTGCAATTGAAAATCTAAG	Xu et al. (2016)
Plasmopara viticola	Rpv 12		SSR	TGAAAGTCGATGGAATGTGC	Venuti et al. (2013)
Plasmopara viticola	Rpv 12		SSR	TTGCATCTCCCTTCTCAATG	Venuti et al. (2013)