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. 2021 May 5;8:642894. doi: 10.3389/fvets.2021.642894

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Copyright © 2021 Okoh, Horwood, Whitmore and Ariel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Midpoint rooted maximum likelihood phylogenetic tree of predicted amino acid sequences of HV-DNA-dependent DNA polymerase. The unassigned reptilian HVs are shown in red and cluster within the subfamily Alphaherpesvirinae. This analysis involved 54 amino acid sequences (37 complete and 17 partial sequences) downloaded from NCBI website (https://www.ncbi.nlm.nih.gov/) and aligned by ClustalW. There were a total of 1,443 positions in the final dataset. The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model. The tree with the highest log likelihood (−60139.09) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Evolutionary analyses were conducted in MEGA X (59).