Figure 1.

BM niche populations identified with current markers

(A) Schematic figure of the experimental design depicting stromal components isolated from the BM of NSG mice carrying specific fluorescent reporters. FACS populations were analyzed by RNA-seq. CD31 n = 7; Nes^high n = 4; Nes^low n = 5; Ng2 n = 3; Osx n = 5; Col^low n = 4; Col^high n = 5.

(B) 3D PCA analysis showing similarities in expression profiles between different stromal components in healthy mice. Each dot represents an experimental replicate.

(C) Flow-cytometry analysis of CD45^–Ter119^– BM cells derived from depicted mice showing overlap between markers associated to niche components.

(D) Correlation matrix showing similarities between different stromal components in healthy mice.

See also Figure S1.