Figure 4.

Progressive detriment of the BM mesenchymal niche in AML

(A) Schematic representation of the experimental setup. Nes^low mesenchymal stromal cells were isolated from the BM of Nestin^GFP-NSG mice xenografted with human cherry⁺-AML cell lines at early and late time points of leukemic engraftment. FACS-sorted populations were analyzed by RNA-seq. Ctrl n = 5; AML early ch⁺ n = 6; AML early ch^– n = 6; AML late n = 6.

(B) Flow cytometry profile of Nes^low cells from control mice or mice engrafted with human cherry⁺-AML cell lines at an early time point.

(C) Pie chart showing the proportion of late deregulated genes also deregulated at early time points.

(D) Heatmap showing the top transcripts deregulated in both the early and late stage of leukemic development in the mesenchymal niche.

(E) Top upregulated (red) and downregulated (blue) processes in the early stage of leukemic engraftment in the mesenchymal niche.

See also Figure S5.