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. 2021 May 18;11:10548. doi: 10.1038/s41598-021-89981-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of Samd1 as an unappreciated transcriptional repressor. (A) Compartmentalization western blot showing distribution of Samd1 in VSMCs. β-actin serves as cytoplasm marker. Lamin A serves as nuclear marker. Cadherin serves as cell membrane marker. (B) Samd1 protein analysis by Phyre2. The Smad1 homologs match has confidence of more than 99.7% and sequence identities of more than 29%. (C) Samd1 predicted domain (left); Polyhomeotic-like protein, chain E (modified PDB 4PZO, middle); Sex comb on midleg cg9495-pa, chain B (modified PDB 1PK1, right). (D) Samd1 secondary structural predication, comparison, analyzation with known DNA binding protein. (E) The pocket detection analysis of Samd1 protein by Fpocket. (F) ChIP assay of Samd1 DNA binding using IgG or anti-Samd1 antibody. Data were presented as mean ± SD, n = 4. *P < 0.05 as compared with IgG control.