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. 2021 May 18;11:10516. doi: 10.1038/s41598-021-90082-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of open reading frames (ORFs) and lncRNAs based on nine full-length transcriptome. (A) Length distribution of ORFs detected in all full-length transcripts. (B) Venn diagram showing the number of lncRNAs identified using four different approaches: CPC (Coding Potential Calculator), CNCI (Coding-Non-Coding Index), CPAT (Coding Potential Assessment Tool), and Pfam (Protein Family). (C) Functional classification and numbers of four lncRNA types. (D) Venn diagram showing the overlap in the differential expressed lncRNAs among the three different developmental stages of G. luofuense male strobili. (E) Representatives of predicted interaction networks among lncRNAs and their target genes. Solid lines and dotted lines represent the expression regulation by the lncRNAs in cis and in trans, respectively. (F) KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotations of cis- and trans-regulated genes regulated by the detected lncRNAs.