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. 2021 May 18;11:10516. doi: 10.1038/s41598-021-90082-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detected differentially expressed transcripts (DETs) and their annotation. (A) Numbers of DETs between different developmental stages of G. luofuense male strobili. (B) Venn diagram showing the overlap in DETs between different developmental stages of male strobili. (C) KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotations of up-regulated transcripts between FA and FC. (D) KEGG pathway annotation of down-regulated transcripts between FA and FC. (E) Two clusters identified based on expression levels of DETs from FA to FC. (F) GO enrichment amongst the two clusters with orange boxes denoting significant enrichment of GO annotation.