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. 2021 May 18;12(6):504. doi: 10.1038/s41419-021-03792-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A qPCR analysis of mRNA levels of M1 macrophage markers in the cells treated with LPS with or without CPS for 24 h. B Western blot and quantitative analysis of iNOS and COX2 proteins in the cells treated with LPS with or without CPS for 48 h. C Immunofluorescence and quantitative analysis of iNOS in RAW264.7 cells treated with LPS with or without CPS for 48 h. D ELISA analysis of IL-6 levels in the supernatant of the culture medium of RAW264.7 cells treated with LPS with or without CPS for 48 h. E Flow cytometry for detection and quantitative analysis of F4/80⁺CD80⁺ cells after the treatment of LPS with or without CPS for 48 h. Data (n = 3) are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars: 25 μm.