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. 2021 May 18;12(6):504. doi: 10.1038/s41419-021-03792-8

Fig. 4. TRPV1 promotes the nuclear translocation of Nrf2 and inhibits M1 macrophage polarization in RAW264.7 cells.

Fig. 4

A Western blot and B quantitative analysis of cytosolic and nuclear Nrf2 protein levels in cells treated with LPS and LPS + CPS with or without ML385 for 3 h. C Immunofluorescence image of Nrf2 and D its quantitative analysis in RAW264.7 cells treated with LPS and LPS + CPS with or without ML385 for 3 h. White arrow: cells with Nrf2 nuclear translocation. E qPCR analysis of mRNA levels of M1 macrophage markers in RAW264.7 cells treated with LPS and LPS + CPS with or without ML385 for 24 h. F Western blot and quantitative analysis of iNOS and COX2 protein expression levels in RAW264.7 cells treated with LPS and LPS + CPS with or without ML385 for 48 h. Data (n = 3) are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars: 10 μm. NS nonsignificant.