Table 1.

Buffer compositions used for various chromatid reconstitution assays.

	Supplementary Fig. 1	Figure 1a	Figure 1b	Figure 1c	Figure 2 afterward
X. laevis topo IIα [nM]	0–250	40	40	40	40
MgCl2 [mM]	2.0	0–5.0	5.0	5.0	5.0
KCl [mM]a	80	120	50–200	80	80 (150)
KH2PO4 [mM]	40	0	0	0	0
ATP [mM]	2.0	2.0	2.0	0–1.0	2.0
HEPES-KOH [mM], pH 7.7	20	20	20	20	20

^aThe KCl concentrations include their carryovers from storage buffers of protein preparations.