TABLE 2.

Allele-specific quantification of BDNF mRNA in human muscle tissue by ddPCR with B2M mRNA as a reference.

ID	Genotype	Allele-specific BDNF mRNA*				B2M/10 mRNA*		BDNF mRNA/1000 B2M mRNA
Condition ->		Pre-Val	Pre-Met	Post-Val	Post-Met	Pre	Post	Pre-Val	Pre-Met	Post-Val	Post-Met
1	Val/Val	1.09	0	3.27	0	83.8	444	1.305	0	0.736	0
2	Val/Val	5.48	0	2.95	0	279	235	1.962	0	1.255	0
3	Val/Met	1.85	1.17	2.08	1.43	220	406	0.840	0.530	0.512	0.346
4	Val/Val	0.62	0	2.64	0	49.6	500	1.253	0	0.528	0
5	Val/Met	1.55	1.11	1.35	1.2	170	268	0.913	0.655	0.503	0.376
6	Val/Val	7.02	0	2.59	0	367	368	1.912	0	0.704	0
7	Val/Val	2.64	0	1.40	0	155	163	1.701	0	0.860	0
8	Val/Val	3.04	0	6.43	0	101.9	435	2.982	0	1.478	0
9	Val/Val	5.17	0	8.47	0	245	714	2.109	0	1.186	0
10	Val/Val	2.70	0	1.80	0	224	302	1.205	0	0.596	0
11	Met/Met	0	1.53	0	1.97	60.8	130	0	2.522	0	1.513
12	Val/Met	0.90	0.63	6.33	5.03	115	1,201	0.783	0.551	0.527	0.419
13	Val/Val	4.40	0	5.43	0	230	428	1.913	0	1.269	0

*Expression values are disposed in (copies/μl of reaction mixture), 1 μl of reaction mixture corresponds to 0.625–1.25 mg of muscle tissue. Pre-Val, BDNF Val66-allele expression values in rest; post-Val, Val66-allele expression values after VO₂max test; pre-Met, BDNF Met66-allele expression values in rest; post-Met, Met66-allele expression values after VO₂max test. B2M, beta-2-microglobulin expression values, pre- and post.