TABLE 5.

Allele-specific quantification of BDNF mRNA in human muscle tissue by ddPCR with RPS18 mRNA as a reference, pre-post VO₂ max test BDNF differences, and Val66-Met66 allele BDNF expression ratio in heterozygotes related to RSP18 mRNA.

ID	Genotype	Allele-specific BDNF mRNA*				RPS18/10 mRNA*		BDNF mRNA/10,000 RPS18 mRNA				BDNF expression decrease (%)		BDNF expression, (Met, % of Val)
Condition ->		Pre-Val	Pre-Met	Post-Val	Post-Met	Pre	Post	Pre-Val	Pre-Met	Post-Val	Post-Met	Val	Met	Pre	Post
1	Val/Val	3.4	0	1.4	0	1,440	1,036	2.36	0	1.35	0	−42.8
2	Val/Val	3.3	0	5	0	1,008	2,566	3.27	0	1.95	0	−40.5
3	Val/Met	2.6	1.7	2.1	1.5	1,006	1,057	2.58	1.69	1.99	1.42	−23.1	−16.0	65.4	71.4
4	Val/Val	3.1	0	0.8	0	1,253	764	2.47	0	1.05	0	−57.7
5	Val/Met	1.7	1.3	1.6	1.4	1,453	1,994	1.17	0.89	0.80	0.70	−31.4	−21.5	76.5	87.5
6	Val/Val	7.9	0	3.4	0	2,322	2,024	3.40	0	1.68	0	−50.6
7	Val/Val	2.2	0	3.3	0	1,088	2,516	2.02	0	1.31	0	−35.1
8	Val/Val	6.5	0	3.1	0	1,203	1,017	5.40	0	3.05	0	−43.6
9	Val/Val	7.5	0	5.7	0	2,361	2,452	3.18	0	2.32	0	−26.8
10	Val/Val	6.4	0	5	0	1,620	1,924	3.95	0	2.60	0	−34.2
11	Met/Met	0	6.1	0	8.8	1,454	3,200	0	4.20	0	2.75	0	−34.5
12	Val/Met	3.8	3.1	2.7	2.4	1,507	2,396	2.52	2.06	1.13	1.00	−55,3	−51.3	81.6	88.9
13	Val/Val	4.1	0	2.9	0	2,275	3,095	1.80	0	0.94	0	−48,0
Mean												−38.3 ± 12.5		78.5 ± 9.2

*Expression values are disposed in (copies/μl of reaction mixture), 1 μl of reaction mixture corresponds to 0.625–1.25 mg of muscle tissue. Pre-Val, BDNF Val66-allele expression values in rest; post-Val, Val66-allele expression values after VO₂max test; pre-Met, BDNF Met66-allele expression values in rest; post-Met, Met66-allele expression values after VO₂max test; RPS18, ribosomal protein S18 gene expression values, pre- and post.