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. 2021 May 18;12:2911. doi: 10.1038/s41467-021-23084-1

Fig. 3. BAFF modifies brown adipose adaptive thermogenesis.

Fig. 3

a–b WT, RP105−/−, or BAFF-Tg mice were fed HFD for 20 weeks. a BAT Ucp1 mRNA expression quantified by qPCR. b BAT mitochondria oxygen consumption. c–d Brown adipocytes were treated with saline (NS) or rBAFF (500 ng/ml) for 6 hours. c Ucp1 mRNA expression quantified by qPCR, relative expression to NS. d Oxygen consumption rate (OCR). e Oxygen consumption of WT mice treated with saline (NS) or rBAFF (2 μg/mouse) for 24 hours prior to norepinephrine (NE; 1 mg/kg) challenge. f–g Obese WT mice treated with rBAFF (2 μg/mouse) every other day for 1 week and monitored in TSE Phenomaster. f Monitoring of energy expenditure over 5 days. g Bar graph of combined energy expenditure. a–b Representative of n = 3–6/condition. c–d Representative of three independent experiments, n = 4–5/condition. e Representative of two independent experiments, n = 3–4/condition. f–g A single experiment, n = 3–5/condition. a–g For bar and line graphs, data represents mean±SEM. a–d Unpaired two-tailed Student’s t test. e Area under the curve. f–g analysis of covariance (ANCOVA) with body weight as covariate. *p < 0.05, ***p < 0.001. Source data are provided as a Source data file.