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. 2021 May 18;11:10476. doi: 10.1038/s41598-021-88448-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Regression analysis of Populations I and II in CD4⁺CD25⁺CD127^loTreg. The proportion of Treg in Populations I and II were analysed for correlation with age of HD and MS patients. (A,B) Linear regression analysis of Populations I with age in HD (n = 20) (A) and MS (n = 36) (B). In HD, Population I decreased with age (R² = 0.542) but not in MS patients (R² = 0.116). (C,D) Linear regression analysis of Populations II with age in HD (n = 20) (C) and MS (n = 36) (D). Population II increased with age for HD (R² = 0.324) and MS (R² = 0.134). (E) Linear regression analysis of Population II with time since last MS relapse showed correlation (R² = 0.143) (p < 0.05).