Skip to main content
. 2021 Apr 16;51:101235. doi: 10.1016/j.molmet.2021.101235

Figure 2.

Figure 2

Analyses of iron-related parameters in the liver. A) DAB-enhanced Perls' iron staining of the liver. B) Quantification of the hepatic non-heme iron content normalized against dry tissue weight. C-D) Gene expression analysis of the iron transferrin-bound iron importer transferrin receptor (TfR)1 (C) and of the iron exporter ferroportin (D). E-F) Western blot analysis of the protein levels of the iron exporter Ferroportin normalized against the housekeeping gene Vinculin. G-K) Gene expression analysis of the iron regulatory hormone hepcidin (G), of the bone morphogenetic protein BMP6 (H), of the BMP/SMAD target genes ID1 (I), SMAD6 (J) and ATOH8 (K) and of the ER-stress related gene CREBH (L) Gene expression mRNA levels have been normalized against the housekeeping gene β-actin (ACTB). All data are reported as mean ± SEM. Six or more mice per group were analyzed. Student's t-test p-value: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.