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. 2021 Apr 16;51:101235. doi: 10.1016/j.molmet.2021.101235

Figure 3.

Figure 3

Characterization of hepatic NAFLD/NASH-related parameters. A-B) Hematoxylin & eosin to detect structural abnormalities and immune cell infiltration (A) and Masson's trichrome stain to detect collaged deposition (B) in livers of 30-week-old female mice with the genotypes indicated C-E). Analysis of the hepatic inflammatory status by measuring gene expression of the inflammatory-related gene SOCS3 (C), of the acute phase protein CRP (D) and of the pro-inflammatory cytokine TNFα (E). F–H) Analysis of hepatic oxidative stress by assessing the mRNA expression of the Nrf2 target gene Nqo1 (F), of lipid peroxidation via the TBARS assay (G) and mRNA expression of the lipid antioxidant enzyme glutathione peroxidase 4 (GPX4) (H). Gene expression values were normalized to the housekeeping gene β-actin (ACTB). Six or more mice per group were analyzed. All data are reported as mean ± SEM. Student's t-test p-value: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.