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. 2021 May 18;12(6):510. doi: 10.1038/s41419-021-03767-9

Fig. 6. CD27-AS1 regulates AML cell activity by directly targeting miR-224-5p.

Fig. 6

a Dual luciferase reporter assay was carried out to verify the binding effects of miR-224-5p on CD27-AS1. Potential binding sequences between them were predicted from Starbase database. b, c Relative miR-224-5p expression was assessed in HL-60 and KG-1 cells with CD27-AS1 overexpression and CD27-AS1 knockdown, respectively, using qRT-PCR. Two AML cells were co-infected with the LV-CD27-AS1 and LV-miR-224-5p. d Cell viability and (e) cell apoptosis were then detected using CCK-8 assay and flow cytometry, respectively. f Western blotting was performed to check protein levels of Ki67, PCNA, BCL-2, cleaved caspase-3, ERK, and p-ERK in the AML cells after co-infection. g Quantification of above protein expression from western blotting. N = 3. Data were shown as means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.