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FITC-d assay work flow for (A) the standard protocol used with normal serum samples (serum pooled from all treatment groups is used as diluent in the standard curve and unknown samples are blanked to the pooled background of all treatments) and (B) the modified protocol for highly autofluorescent serum samples (per treatment background autofluorescence subtracted from each unknown serum sample, respective to treatment group, and standard curve constructed with saline as diluent).
