Fig. 6.

Disaggregation of Aβ1-42 by Mimo-AuNPs protects cultured neurons. (a & b) Histogram depicting the dose-dependent decrease in the viability of mouse cortical cultured neurons following 24h treatment with oligomeric human Aβ1-42 compared to neurons treated with 10 μM Aβ42-1 (Cont) as revealed by MTT and LDH assays. (c & d) Histograms showing protection of cortical neurons following disassembly of preformed Aβ1-42 aggregates by Mimo-AuNPs as detected with MTT (c) and LDH (d) assays. (e–l) Immunoblots and corresponding histograms showing that the protective effect of Mimo-AuNPs is mediated by decreasing the levels of Phospho-ERK1/2 (e, f), Phospho-Tyr216 GSK-3β (g, h), Phospho-tau (i, j) and carbonylated protein (k, l) levels induced by preformed aggregated Aβ1-42. All data expressed as mean ± SEM were obtained from three to five separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.