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. 2021 May 18;11:10495. doi: 10.1038/s41598-021-89860-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IL-4 stimulation downregulates CD28 and IFN-γR1 expression on CD4 T cells. Whole spleen homogenate derived from naïve 6–8-week-old female BALB/c mice (n = 4) were stimulated with 50 ng/mL IL-4 for 24 h. (a) Representative histograms showing CD28 expression on pre-gated CD4 T cells partitioned based on their IL-4R⍺ status. (b) MFI (CD28) for each treatment condition. (c) Representative contour plots showing the expression of IFN-γR1 on pre-gated CD4 T cells for each CD62L:CD44 status. (d) The proportion of cells deemed IFN-γR1⁺. Data are represented as mean + SD, where dots represent biological replicates from a single experiment. The experiments were repeated at least twice. Two-way ANOVA combined with Tukey’s post-hoc multiple comparison test was conducted to compare groups. p-value denotation: ‘****’ p < 0.0001.