Table 2.

miR-based therapy and their role as antimetastatic drugs in BC treatment.

miR-based treatment	miR target	Methodological approach	In vitro BC model tested	Gene(s)/pathway regulated	In vivo BC model tested	Delivery system(administration route)	Biological effect	Ref.
miR-inhibition therapy	miR-10b	antagomiR-10b	4T1	–	Orthotopic mouse (4T1)	Transfection and intravenously injection	Suppressed formation of lung cancer metastasis	Ma et al. (27)
	miR-10b	LNA-based antagomiR-10b	MDA-231-BoM-1833, MDA-231-LM2-4175, MDA-231-BrM2-831, MDA-MB-231-luc-D3H2LN	HOXD10	Orthotopic mouse (4T1)	Iron oxide NPs and intravenously injection	reduced pre-existing distant metastases and cancer mortality	Yoo et al. (28)
	miR-10b	antagomiR-10b	4T1	HOXD10	Orthotopic mouse (4T1)	Liposomes (PEG-Lip) intravenously injection	Delayed the growth of the primary tumor and reduced the lung metastases	Zhang et al. (29)
	miR-214	antagomiR-214 (R97 or R98)	4175-TGL	–	Orthotopic mouse (4175-TGL)	Unassisted uptake (chemically modified miR) and intravenously injection	Reduced number of circulating tumor cells and lung or lymph node metastasis formation	Dettori et al. (30)
	miR-222, miR-223	antagomiR-222/223	MDA-MB-231, T47D	–	Xenograft mouse (MDA-MB-231 or T47D)	Transfection and intravenously injection	sensitized BC to carboplatin-based therapy and increased survival	Bliss et al. (31)
	miR-205-5p	LNA-based antagomiR-205-5p	cell from tissue patients	–	Xenograft mouse (BC stem cells)	Unassisted uptake (chemically modified miR) and intraperitoneally injection	Impaired tumor growth and reduced the number and the size of lung metastasis	De Cola et al. (32)
	miR-100	antagomiR-100	4T1, RAW264.7	mTOR/Stat5a pathway	Orthotopic mouse (4T1)	Unassisted uptake (chemically modified miR) and intratumorally injection	Inhibited lung metastasis and enhanced chemotherapy-sensitivity	Wang et al. (33)
miR-replacement therapy	miR-4306	miR-4306 mimic	ZR-75-1, MCF-7, T47D, SK-BR-3, HCC1937, MDA-MB-468, MDA-MB-231, CAL-51	SIX1, Cdc42, VEGFA	Orthotropic xenograft mouse (CAL-51)	Cholesterol-conjugated and intratumorally injection	Inhibited TNBC cell growth, lung metastasis, angiogenesis and lymphatic metastasis	Zhao et al. (34)
	miR-708	miR-708 mimic	293, 4T1 and MDA-MB-231	–	Orthotopic mouse (MDA-MB-231-LM2)	Multilayer AuNPs and intratumorally injection	reduced lung metastasis formation	Ramchandani et al. (35)
	miR-34a	tRNA-miR-34a	4T1.2, MDA-MB-231	–	Orthotopic mouse (4T1.2)	Multifunctional polymeric nano micelles and intravenously injection	Inhibited metastasis formation and enhanced antitumoral immune response	Xu et al. (36)
	miR-36133-3p	miR-3613-3p mimic	MCF7, MDA-MB-231	SMS, PAFAH1B2, or PDK3	Xenograft mouse (MDA-MB-231)	Transfection and subcutaneously injection	Reduced the degree of pulmonary metastasis formation and primary tumor volume	Chen et al. (37)