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. 2021 May 5;12:628301. doi: 10.3389/fmicb.2021.628301

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Copyright © 2021 Suominen, van Vliet, Sánchez-Andrea, van der Meer, Sinninghe Damsté and Villanueva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of active operational taxonomic units (OTUs) and their taxonomy compared to all OTUs. Data shown is ordered by substrate type and sampling timepoints day 7 (T7), day 21 (T21), and day 63 (T63). The x-axis depicts the difference in abundance between the heavy fractions of the labeled and unlabeled incubations as the log2 fold change calculated with DeSeq2 in the high-resolution DNA-SIP (HRSIP) workflow as indicated in the Experimental procedures. (A) Acetate-amended incubations, (B) Protein-amended incubations, and (C) PAOM-amended incubations. Each dot represents an individual OTU. Light gray dots represent unlabeled OTUs, while colored dots are OTUs were determined as labeled by the HRSIP analysis. Dots are color-coded by taxonomic information with colors corresponding to the ones used in Figure 2.