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SNP272 effect on mRNA stability. HEK293 cells were transfected with EDN2-3′-UTR luciferase constructs (SNP272 A/G). Luciferase activities (in triplicates) were measured 24 h post-transfection. Renilla luciferase activities were normalized against firefly luciferase activities, and mean normalized Renilla luciferase activities (± SD) from three independent experiments were determined. There was no difference (P > 0.05) in luciferase activities between the A and G alleles at SNP272.
