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. 2021 May 5;8:625323. doi: 10.3389/fvets.2021.625323

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Copyright © 2021 Kiser, Wang, Zanella, Scraggs, Neupane, Cantrell, Van Tassell, White, Taylor and Neibergs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) SNP208 Luciferase reporter assay. HEK293 cells were transfected with EDN2 promoter-pGL3 reporter constructs containing either G or T at the location of SNP208. The relative luciferase activities were calculated and expressed as mean ± SD. The relative luciferase activity of the genotype G at SNP208 was significantly higher (P = 0.01) compared with that of the genotype T. (B) SNP105 and SNP208 interaction. HEK293 cells were transfected with EDN2 promoter-pGL3 reporter constructs containing different combination of SNP105(A/G) and SNP208(G/T). The relative luciferase activities were calculated and normalized to construct 105G/208G. The mean of relative luciferase activity (±SD) from three independent experiments was shown. The relative luciferase activity of the G genotype at SNP208 and the A genotype at SNP105 was significantly higher (P = 0.02) than genotype combinations.