Figure 2.

FUS forms complexes with TNPO3, importin β, importin 7, and importin β/7. MBP–FUS–EGFP was incubated with different His-tagged nuclear transport receptors in the absence (A, C, and E–I) or presence (B, D, and J) of RanQ69L(aa1-180)–GTP and subjected to size-exclusion chromatography, monitoring UV absorbance at 280 nm. Eluate fractions were analyzed by SDS-PAGE, followed by Coomassie staining. Note that FUS alone was not soluble after a 100,000g centrifugation step and was therefore not detected by size-exclusion chromatography.