Figure 3.

The C terminus of importin 7 is required for the formation of a trimeric FUS–importin β–importin 7 complex.A–D, different combinations of MBP–FUS–EGFP, His–importin β, His–importin 7wt, and His–importin 7ΔC (aa1-1001) were incubated and subjected to size-exclusion chromatography, monitoring UV absorbance at 280 nm. Eluate fractions were analyzed by SDS-PAGE, followed by Coomassie staining. Note that FUS alone was not soluble after a 100,000g centrifugation step and was therefore not detected by size-exclusion chromatography.