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. 2021 Apr 12;296:100659. doi: 10.1016/j.jbc.2021.100659

Figure 8.

Figure 8

Nuclear import of FUS in digitonin-permeabilized cells by TNPO1 and alternative NTRs. Digitonin-permeabilized HeLa cells were incubated with MBP–FUS in the absence or presence of different His-tagged nuclear transport receptors, in the absence or presence of an ATP-regenerating system, with either WT Ran or RanQ69L (Q69L) at 30 °C or 4 °C as indicated. A, cells were subjected to indirect immunofluorescence detecting the MBP tag and analyzed by confocal microscopy. The scale bar represents 20 μm. B, quantification of the mean nuclear import efficiencies of FUS as in panel A for four or three (“-”, 30 °C, importin β, 30 °C) independent experiments ± SEM (25–70 cells per condition).