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. 2021 Mar 12;8(10):2004705. doi: 10.1002/advs.202004705

Figure 3.

Figure 3

Role of hyaluronan in HELP matrices. a) Flow cytometric analysis of enteroids grown in EHS and HELP matrices 11 days post‐encapsulation for CD44‐positive cells, compared to negative controls, dashed line indicates flow gating. b) Quantification of percentages of CD44‐positive cells in EHS matrix and HELP across three independent experiments. c) Gene expression of CD44 for passage 20 enteroids cultured in HELP for different lengths of time and EHS matrix (all three conditions had 20 total passages, normalized to P20 in EHS). One‐way ANOVA with Tukey post‐hoc testing, n = 4, * = p < 0.05, *** = p < 0.001. d) Storage moduli for HELP and ELP‐PEG formulations as measured by oscillatory rheology. At least three measurements were performed for each material. Two‐tailed Student's t‐test, n.s. = not significant. e) Brightfield and confocal micrographs at 6 days post‐seeding of enteroids grown in HELP and ELP‐PEG matrices, where HA is absent in the ELP‐PEG gels. f) Formation efficiency between enteroids grown in HELP and ELP‐PEG. Two‐tailed Student's t‐test, ** = p < 0.01, n = 3, n.d. = none detected. Data shown are mean ± SD.