Skip to main content
. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: Methods Mol Biol. 2019;1982:191–229. doi: 10.1007/978-1-4939-9424-3_12

Fig. 1.

Fig. 1

Preventing contamination in RT-qPCR. (a) Wash the bench and surfaces of tools and equipment with a paper towel soaked in diluted detergent and water. If possible unscrew the lower parts of the pipette barrel and wash in soapy water. (b) The dial and top of the pipette where the thumb is placed is a large source of contamination. (c) Treat the washed pipettes and test tube racks with UV light in a biosafety cabinet or in a UV light box for 1 h. (d) Use a gDNA eliminator column twice to ensure gDNA removal